General Science MCQ – 152 (Biology)

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1. DNA base pairs in the human genome are about?
(A) 2 X 10⁷
(B) 3 X 10⁹
(C) 4 X 10⁸
(D) 7 X 10⁷

2. Which of the following is a dual resistance vector?
(A) M13
(B) pUC 18
(C) pUC 19
(D) pBR 322

3. The name Kary Mullis is associated with?
(A) Gel Retardation Assay
(B) Chain Termination Reaction
(C) RFLP
(D) PCR

4. Alec Jeffery’s name is associated with?
(A) DNA sequencing
(B) RNA sequencing
(C) DNA fingerprinting
(D) Site-directed mutagenesis

5. The Cre-Lox system uses one of the following?
(A) DNA kinase
(B) Creatine recombinase
(C) DNA recombinase
(D) S1-nuclease

6. Yeast two-hybrid system involves the use of?
(A) Domains of transcription factors
(B) Histone proteins
(C) Two-point crossovers
(D) Homologous recombination

7. The method of plasmid isolation by alkaline-lysis was published by?
(A) Mandel and Higa
(B) Temin and Baltimore
(C) Tatum and Lederberg
(D) Brinboim and Doly

8. The antisense gene involved in the production of Flavr Savr tomato is?
(A) Polygalacturonase
(B) Lactamase
(C) Adenosine deaminase
(D) Glutathione transferase

9. The genetically engineered ‘Golden Rice synthesizes a large amount of?
(A) Vitamin K
(B) Beta-carotene
(C) Vitamin C
(D) Beta-galactosidase

10. His-tagged proteins can be eluted from Ni columns using?
(A) Imidazole
(B) Ammonium hydroxide
(C) 6X SSC
(D) 0.25 N HCl

General Science MCQ – 151 (Physics)